Evaluation of a virus derived from MDCK cells infected persistently with influenza A virus as a potential live‐attenuated vaccine candidate in the mouse model
Identifieur interne : 001332 ( Main/Exploration ); précédent : 001331; suivant : 001333Evaluation of a virus derived from MDCK cells infected persistently with influenza A virus as a potential live‐attenuated vaccine candidate in the mouse model
Auteurs : Beixing Liu [Japon, République populaire de Chine] ; Md. Jaber Hossain [Japon] ; Isamu Mori [Japon] ; Yoshinobu Kimura [Japon]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2008-05.
English descriptors
- Teeft :
- Amino, Amino acid, Amino acid change, Amino acid changes, Amino acid sequences, Amino acids, Cambridge strain, Cellular distribution, Challenge virus, Disease control, Fukui university school, Gifu university, Glass coverslips, Higher temperatures, Immune responses, Incubation temperatures, Infection, Infectious progeny virus, Inoculated intranasally, Inoculum dose, Intranasal infection, Intranasal inoculation, Ivwt, Ivwt virus, Maintenance medium, Matrix, Matrix gene segment, Matrix protein, Mdck, Mdck cells, Medical science, Medical technology, Monoclonal antibodies, Monoclonal antibody, Mouse, Mutant, Mutant virus, Nuclear export, Nuclear import, Nuclear transport, Nucleotide, Nucleotide sequence, Observation period, Persistent infection, Plaque titration, Present study, Progeny virus, Small dose, Spleen cells, Target cells, Vaccinated mice, Vaccine, Virol, Virol tobita, Virus, Virus carrier culture, Virus challenge, Virus growth, Virus infectivity, Virus proteins, Virus replication.
Abstract
A temperature‐sensitive mutant virus unable to replicate at 38°C was recovered from passage 189 (IVpi‐189) of Madin‐Darby canine kidney cells infected persistently with influenza A. Immunofluorescent staining of the IVpi‐189 virus‐infected cells revealed disrupted transport of the matrix (M) 1 protein into the nucleus at non‐permissive temperatures, resulting in retention of the nucleoprotein (NP) in the nucleus. Upon comparison with the parental influenza A E61‐24‐P15 strain used to establish persistent infection, amino acid exchanges were found in the M1 protein of IVpi‐189 virus; arginine to glutamine at position 72 and threonine to alanine at position 139. When mice were inoculated intranasally with IVpi‐189 virus, virus growth in the lungs was restrained and terminated rapidly. Prior intranasal inoculation with only a small dose of IVpi‐189 virus induced humoral and cellular immune responses and protected mice against subsequent virulent virus challenge. These results indicate that IVpi‐189 virus, an avirulent temperature‐sensitive mutant, is a promising candidate for use as a live‐attenuated vaccine. J. Med. Virol. 80:888–894, 2008. © 2008 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.21148
Affiliations:
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Immunofluorescent staining of the IVpi‐189 virus‐infected cells revealed disrupted transport of the matrix (M) 1 protein into the nucleus at non‐permissive temperatures, resulting in retention of the nucleoprotein (NP) in the nucleus. Upon comparison with the parental influenza A E61‐24‐P15 strain used to establish persistent infection, amino acid exchanges were found in the M1 protein of IVpi‐189 virus; arginine to glutamine at position 72 and threonine to alanine at position 139. When mice were inoculated intranasally with IVpi‐189 virus, virus growth in the lungs was restrained and terminated rapidly. Prior intranasal inoculation with only a small dose of IVpi‐189 virus induced humoral and cellular immune responses and protected mice against subsequent virulent virus challenge. These results indicate that IVpi‐189 virus, an avirulent temperature‐sensitive mutant, is a promising candidate for use as a live‐attenuated vaccine. J. Med. Virol. 80:888–894, 2008. © 2008 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Japon</li><li>République populaire de Chine</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Liu, Beixing" sort="Liu, Beixing" uniqKey="Liu B" first="Beixing" last="Liu">Beixing Liu</name></noRegion><name sortKey="Hossain, Md Jaber" sort="Hossain, Md Jaber" uniqKey="Hossain M" first="Md. Jaber" last="Hossain">Md. Jaber Hossain</name><name sortKey="Kimura, Yoshinobu" sort="Kimura, Yoshinobu" uniqKey="Kimura Y" first="Yoshinobu" last="Kimura">Yoshinobu Kimura</name><name sortKey="Kimura, Yoshinobu" sort="Kimura, Yoshinobu" uniqKey="Kimura Y" first="Yoshinobu" last="Kimura">Yoshinobu Kimura</name><name sortKey="Kimura, Yoshinobu" sort="Kimura, Yoshinobu" uniqKey="Kimura Y" first="Yoshinobu" last="Kimura">Yoshinobu Kimura</name><name sortKey="Kimura, Yoshinobu" sort="Kimura, Yoshinobu" uniqKey="Kimura Y" first="Yoshinobu" last="Kimura">Yoshinobu Kimura</name><name sortKey="Mori, Isamu" sort="Mori, Isamu" uniqKey="Mori I" first="Isamu" last="Mori">Isamu Mori</name><name sortKey="Mori, Isamu" sort="Mori, Isamu" uniqKey="Mori I" first="Isamu" last="Mori">Isamu Mori</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Beixing" sort="Liu, Beixing" uniqKey="Liu B" first="Beixing" last="Liu">Beixing Liu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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